For a first visit, three to four full days is the sweet spot: one for Ancient Rome, one for the Vatican, and one or two for the historic center, Trastevere and Roman food.
Two days can cover Ancient Rome, the Vatican and the historic center at a brisk pace, but it leaves little room for neighborhoods and long meals.
Add a day for Tivoli or Ostia Antica. Florence is better as a two-day extension, and the Amalfi Coast deserves at least two or three days.
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